Tetrodotoxin-sensitive sodium current in sheep lymphatic smooth muscle.

Hollywood, Mark A. and Cotton, K. D. and Thornbury, Keith D. and McHale, Noel G. (1997) Tetrodotoxin-sensitive sodium current in sheep lymphatic smooth muscle. J Physiol. , 503 (1). pp. 13-20.

[thumbnail of Tetrodot.pdf]
PDF - Published Version
Download (1MB) | Preview


Fast inward currents were elicited in freshly isolated sheep lymphatic smooth muscle cells by depolarization from a holding potential of -80 mV using the whole-cell patch-clamp technique. The currents activated at voltages positive to -40 mV and peaked at 0 mV. 2. When sodium chloride in the bathing solution was replaced isosmotically with choline chloride inward currents were abolished at all potentials. 3. These currents were very sensitive to tetrodotoxin (TTX). Peak current was almost abolished at 1 microM with half-maximal inhibition at 17 nM. 4. Examination of the voltage dependence of steady state inactivation showed that more than 90% of the current was available at the normal resting potential of these cells (-60 mV). 5. The time course of recovery from inactivation was studied using a double-pulse protocol and showed that recovery was complete within 100 ms with a time constant of recovery of 20 ms. 6. Under current clamp, action potentials were elicited by depolarizing current pulses. These had a rapid upstroke and a short duration and could be blocked with 1 microM TTX. 7. Spontaneous contractions of isolated rings of sheep mesenteric lymphatic vessels were abolished or significantly depressed by 1 microM TTX.

Item Type: Article
Uncontrolled Keywords: Action Potentials/drug effects; Animals; Choline/metabolism; Female; Lymphatic System/drug effects; Lymphatic System/physiology; Male; Membrane Potentials/drug effects; Membrane Potentials/physiology; Muscle, Smooth/drug effects; Muscle, Smooth/physiology; Nickel/pharmacology; Nifedipine/pharmacology; Patch-Clamp Techniques; Sheep; Sodium Channels/drug effects; Sodium Channels/physiology; Tetrodotoxin/pharmacology
Subjects: Science
Research Centres: Smooth Muscle Research Centre
Depositing User: Concepta Woods
Date Deposited: 08 Mar 2012 16:35
Last Modified: 11 Nov 2014 16:10

Actions (login required)

View Item View Item


Downloads per month over past year